Exhibit 16.1

Tel: +61 7 3237 5999 Fax: +61 7 3221 9227 www.bdo.com.au

Level 18, 360 Queen Street Brisbane QLD 4000 GPO Box 457 Brisbane QLD 4001 Australia

Letter to Confirming Termination of Client-Auditor Relationship

11 March 2026

Mr. Gary Seaton, Board Chairman

Mr. Long Yi, Audit Committee Chairman

Mr. Saw Khoon Ming, Co-Chief Executive Officer

Mr. Amarjeet Singh, Chief Financial Officer

Australian Oilseeds Holdings Limited

126 – 142 Cowcumbla Street

Cootamundra, NSW, Australia

Dear Mr. Seeton, Mr. Yi, Mr. Koon Ming, and Mr. Singh

This is to confirm that the client-auditor relationship between Australian Oilseeds Holdings Limited and BDO Audit Pty Ltd has ceased.

Sincerely,

BDO Audit Pty Ltd

Nigel Batters

Director

Office of the Chief Accountant

Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549-7561

BDO Audit Pty Ltd ABN 33 134 022 870 is a member of a national association of independent entities which are all members of BDO International Ltd, a UK company limited by guarantee, and form part of the international BDO network of independent member firms. Liability limited by a scheme approved under Professional Standards Legislation.